Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth

Title
Black ginseng activates Akt signaling, thereby enhancing myoblast differentiation and myotube growth
Authors
김수남이설림이수연고가연Tuan Anh Vuong김지원조아영안준민서동완김진석김용기강종선이상진배규운
Issue Date
2018-01
Publisher
Journal of Ginseng Research
Citation
VOL 42, NO 1-121
Abstract
Background Black ginseng (BG) has greatly enhanced pharmacological activities relative to white or red ginseng. However, the effect and molecular mechanism of BG on muscle growth has not yet been examined. In this study, we investigated whether BG could regulate myoblast differentiation and myotube hypertrophy. Methods BG-treated C2C12 myoblasts were differentiated, followed by immunoblotting for myogenic regulators, immunostaining for a muscle marker, myosin heavy chain or immunoprecipitation analysis for myogenic transcription factors. Results BG treatment of C2C12 cells resulted in the activation of Akt, thereby enhancing heterodimerization of MyoD and E proteins, which in turn promoted muscle-specific gene expression and myoblast differentiation. BG-treated myoblasts formed larger multinucleated myotubes with increased diameter and thickness, accompanied by enhanced Akt/mTOR/p70S6K activation. Furthermore, the BG treatment of human rhabdomyosarcoma cells restored myogenic differentiation. Conclusion BG enhances myoblast differentiation and myotube hypertrophy by activating Akt/mTOR/p70S6k axis. Thus, our study demonstrates that BG has promising potential to treat or prevent muscle loss related to aging or other pathological conditions, such as diabetes.
URI
http://pubs.kist.re.kr/handle/201004/67550
ISSN
1226-8453
Appears in Collections:
KIST Publication > Article
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