Neuronal differentiation of human mesenchymal stem cells in response to the domain size of graphene substrates

Title
Neuronal differentiation of human mesenchymal stem cells in response to the domain size of graphene substrates
Authors
김명종서태훈이유정이슬아장원희성정석
Keywords
graphene; defects; stem cells
Issue Date
2018-01
Publisher
Journal of biomedical materials research. Part A
Citation
VOL 106A-51
Abstract
Graphene is a noncytotoxic monolayer platform with unique physical, chemical, and biological properties. It has been demonstrated that graphene substratemay provide a promising biocompatible scaffold for stem cell therapy. Because chemical vapor deposited graphene has a two dimensional polycrystalline structure, it is important to control the individual domain size to obtain desirable properties for nano-material. However, the biological effects mediated by differences in domain size of graphene have not yet been reported. On the basis of the control of graphene domain achieved by one-step growth (1step-G, small domain) and two-step growth (2step-G, large domain) process, we found that the neuronal differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) highly depended on the graphene domain size. The defects at the domain boundaries in 1step-G graphene was higher (38.5) and had a relatively low (13% lower) contact angle of water droplet than 2step-G graphene, leading to enhanced cellsubstrate adhesion and upregulated neuronal differentiation of hMSCs.We confirmed that the strong interactions between cells and defects at the domain boundaries in 1step-G graphene can be obtained due to their relatively high surface energy, which is stronger than interactions between cells and graphene surfaces. Our results may provide valuable information on the development of graphene-based scaffold by understanding which properties of graphene domain influence cell adhesion efficacy and stem cell differentiation.
URI
http://pubs.kist.re.kr/handle/201004/67687
ISSN
1549-3296
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE