A Simple Colorimetric Method to Quantify Total Fecal Oil Using Oil-soluble Dyes in Laboratory Animals and Its Correlation with Liquid Chromatography?Mass Spectrometry Analysis

Title
A Simple Colorimetric Method to Quantify Total Fecal Oil Using Oil-soluble Dyes in Laboratory Animals and Its Correlation with Liquid Chromatography?Mass Spectrometry Analysis
Authors
이정애정혜선장유라
Keywords
fecal oil; colorimetry; sudan dyes
Issue Date
2018-05
Publisher
Analytical sciences
Citation
VOL 34, NO 5-626
Abstract
We developed a colorimetric method for measuring the amount of oil in mouse stool after co-administering an oil-soluble dye. When the amount of oil in the feces calculated from the amounts of Sudan III and Oil Red O was plotted against the amount of oil detected by liquid chromatography– mass spectrometry, the graph was linear showing one-to-one correlation between two analyses. This method may be utilized to determine the efficacy of lipase inhibitors or to assess fat malabsorption in vivo.
URI
http://pubs.kist.re.kr/handle/201004/67840
ISSN
0910-6340
Appears in Collections:
KIST Publication > Article
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