A Simple Colorimetric Method to Quantify Total Fecal Oil Using Oil-soluble Dyes in Laboratory Animals and Its Correlation with Liquid Chromatography？Mass Spectrometry Analysis
- A Simple Colorimetric Method to Quantify Total Fecal Oil Using Oil-soluble Dyes in Laboratory Animals and Its Correlation with Liquid Chromatography？Mass Spectrometry Analysis
- 이정애; 정혜선; 장유라
- fecal oil; colorimetry; sudan dyes
- Issue Date
- Analytical sciences
- VOL 34, NO 5-626
- We developed a colorimetric method for measuring the amount of oil in mouse stool after co-administering an oil-soluble dye. When the amount of oil in the feces calculated from the amounts of Sudan III and Oil Red O was plotted against the amount of oil detected by liquid chromatography– mass spectrometry, the graph was linear showing one-to-one correlation between two analyses. This method may be utilized to determine the efficacy of lipase inhibitors or to assess fat malabsorption in vivo.
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