Discrimination of Avian Influenza Virus Subtypes using Host-Cell Infection Fingerprinting by a Sulfinate-based Fluorescence Superoxide Probe
- Discrimination of Avian Influenza Virus Subtypes using Host-Cell Infection Fingerprinting by a Sulfinate-based Fluorescence Superoxide Probe
- 우덕하; 이석; 이준석; 김윤경; 서민아; 마무눌 하크; 디라제무라레; 홍성철; 장세영; 권정훈; 송창선
- Avian influenza; fluorescent probe
- Issue Date
- Angewandte Chemie international edition
- VOL 57, NO 31-9721
- The current gold-standard diagnosis method for avian influenza (AI) is an embryonic egg-based hemagglutination assay followed by immunoblotting or PCR sequencing to confirm subtypes. It requires, however, specialized facilities to handle egg inoculation and incubation, and the subtyping methods relied on costly reagents. Now, the first differential sensing approach to distinguish AI subtypes is demonstrated using series of cell lines and a fluorescent sensor. Susceptibility of AI virus differs depending on genetic backgrounds of host cells. Cells were examined from different organ origins, and the infection patterns against a panel of cells were utilized for AI virus subtyping. To quantify AI infection, a highly cellpermeable fluorescent superoxide sensor was designed to visualize infection. This differential sensing strategy successfully
proved discriminations of AI subtypes and demonstrated as a useful primary screening platform to monitor a large number of samples.
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