Structural Modification of (-)-Epigallocatechin Gallate (EGCG) Shows Significant Enhancement in Mitochondrial Biogenesis
- Structural Modification of (-)-Epigallocatechin Gallate (EGCG) Shows Significant Enhancement in Mitochondrial Biogenesis
- 추현아; 하태웅; 김미경; 박광수; 정웅; 정유훈
- (-)-EGCG; structural modification; mitochondrial biogenesis; antiaging
- Issue Date
- Journal of agricultural and food chemistry
- VOL 66, NO 15-3859
- (-)-Epigallocatechin-3-gallate (EGCG) is known as a mitochondria-targeted molecule that can prevent mitochondrial deterioration and induce mitochondrial biogenesis by modulating key regulators of mitochondria' metabolism. In this study, we tackled whether derivatization of EGCG could result in enhancement of its effects on mitochondrial biogenesis. EGCG, EGCG peracetate (AcEGCG), and its 4 ''-O-alkyl substituted congeners prepared by previously reported procedures were biologically evaluated. Interestingly, EGCG and AcEGCG were only marginally effective in inducing mitochondrial biogenesis, while AcEGCG congeners with an alkyl group at the 4 ''-O position showed significantly increased biological activity compared to their parent compound. Among these series, 3f with a methyl-branched carbonate chain at the 4 ''-O position of the AcEGCG scaffold showed the most enhancement in inducing mitochondrial biogenesis. Hepal 6 cells treated with 3f exhibited increases in both mitochondria' mass (1.5 times) and relative mtDNA content to nDNA (1.5 times). As a mitochondrial biogenesis enhancer, 3f also increased expression levels of regulators for mitochondria' function, induding PGC-1 alpha (4.0 fold), p-AMPK (2.5 fold), SIRT1 (4.2 fold), ERRa (1.8 fold), NRF-1 (1.6 fold), NRF-2 (1.7 fold), and mtTFA (1.6 folds). Investigation of oxidative phosphorylation by mitochondria in the presence of 3f revealed that 3f increased the NADVNADH ratio, the amount of cytochrome c, ATP synthesis, and oxygen consumption in Hepal 6 cells by 2.2, 1.4, 1.5, and 2.1 fold, respectively. Taken together, these results warrant an extensive structure activity relationship study for EGCG derivatives to develop novel mitochondrial biogenesis enhancers.
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