Streptavidin-mirror DNA tetrahedron hybrid as a platform for intracellular and tumor delivery of enzymes

Title
Streptavidin-mirror DNA tetrahedron hybrid as a platform for intracellular and tumor delivery of enzymes
Authors
권익찬안대로정학숙김경란김주현황두현이창용이원석윤대성신동윤민선준
Keywords
Enzyme delivery; cancer therapy; DNA tetrahedron; Streptavidin-mirror DNA tetrahedron hybrid
Issue Date
2018-06
Publisher
Journal of controlled release
Citation
VOL 280-10
Abstract
Despite the extremely high substrate specificity and catalytically amplified activity of enzymes, the lack of efficient cellular internalization limits their application as therapeutics. To overcome this limitation and to harness enzymes as practical biologics for targeting intracellular functions, we developed the streptavidin-mirror DNA tetrahedron hybrid as a platform for intracellular delivery of various enzymes. The hybrid consists of streptavidin, which provides a stoichiometrically controlled loading site for the enzyme cargo and an L-DNA (mirror DNA) tetrahedron, which provides the intracellular delivery potential. Due to the cell-penetrating ability of the mirror DNA tetrahedron of this hybrid, enzymes loaded on streptavidin can be efficiently delivered into the cells, intracellularly expressing their activity. In addition, we demonstrate tumor delivery of enzymes in an animal model by utilizing the potential of the hybrid to accumulate in tumors. Strikingly, the hybrid is able to transfer the apoptotic enzyme specifically into tumor cells, leading to strong suppression of tumor growth without causing significant damage to other tissues. These results suggest that the hybrid may allow anti-proliferative enzymes and proteins to be utilized as anticancer drugs.
URI
http://pubs.kist.re.kr/handle/201004/68190
ISSN
0168-3659
Appears in Collections:
KIST Publication > Article
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