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dc.contributor.authorSo Young Chun-
dc.contributor.authorDae Hwan Kim-
dc.contributor.authorJeong Shik Kim-
dc.contributor.authorHyun Tae Kim-
dc.contributor.authorEun Sang Yoo-
dc.contributor.authorJae-Wook Chung-
dc.contributor.authorYun-Sok Ha-
dc.contributor.authorPhil Hyun Song-
dc.contributor.authorSung Kwang Chung-
dc.contributor.authorBum Soo Kim-
dc.contributor.authorTae Gyun Kwon-
dc.identifier.citationVOL 15, NO 4-466-
dc.description.abstractKidney ischemia-reperfusion (IR) via laparotomy is a conventional method for kidney surgery in a mouse model. However, IR, an invasive procedure, can cause serious acute and chronic complications through apoptotic and inflammatory pathways. To avoid these adverse responses, a Non-IR and dorsal slit approach was designed for kidney surgery. Animals were divided into three groups, 1) sham-operated control-
dc.description.abstract2) IR, Kidney IR via laparotomy-
dc.description.abstractand 3) Non-IR, Non-IR and dorsal slit. The effects of Non-IR method on renal surgery outcomes were verified with respect to animal viability, renal function, apoptosis, inflammation, fibrosis, renal regeneration, and systemic response using histology, immunohistochemistry, real-time polymerase chain reaction, serum chemistry, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, and Masson's trichrome staining. The Non-IR group showed 100% viability with mild elevation of serum blood urea nitrogen and creatinine values at day 1 after surgery, whereas the IR group showed 20% viability and lethal functional abnormality. Histologically, renal tubule epithelial cell injury was evident on day 1 in the IR group, and cellular apoptosis enhanced TUNEL-positive cell number and Fas/caspase-3 and KIM-1/NGAL expression. Inflammation and fibrosis were high in the IR group, with enhanced CD4/CD8-positive T cell infiltration, inflammatory cytokine secretion, and Masson's trichrome stain-positive cell numbers. The Non-IR group showed a suitable microenvironment for renal regeneration with enhanced host cell migration, reduced immune cell influx, and increased expression of renal differentiation-related genes and anti-inflammatory cytokines. The local renal IR influenced distal organ apoptosis and inflammation by releasing circulating pro-inflammatory cytokines. The Non-IR and dorsal slit method for kidney surgery in a mouse model can be an alternative surgical app-
dc.publisherTissue Engineering and Regenerative Medicine-
dc.titleA Novel Dorsal Slit Approached Non-Ischemic Partial Nephrectomy Method for a Renal Tissue Regeneration in a Mouse Model-
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