Transgenic zebrafish model for quantification and visualization of tissue toxicity caused by alloying elements in newly developed biodegradable metal

Title
Transgenic zebrafish model for quantification and visualization of tissue toxicity caused by alloying elements in newly developed biodegradable metal
Authors
이관희석현광김유찬한형섭박지민서현선Gun Hyuk JangIndong JunSion Glyn-JonesDiego MantovaniJames R. Edwards
Keywords
Transgenic zebrafish model; tissue toxicity; biodegradable metal
Issue Date
2018-09
Publisher
Scientific Reports
Citation
VOL 8-13818-9
Abstract
The cytotoxicity of alloying elements in newly developed biodegradable metals can be assessed through relatively low-cost and rapid in vitro studies using different cell types. However, such approaches have limitations; as such, additional investigations in small mammalian models are required that recapitulate the physiological environment. In this study, we established a zebrafish (Danio rerio) model for cytotoxicity evaluations that combines the physiological aspects of an animal model with the speed and simplicity of a cell-based assay. The model was used to assess the cytotoxicity of five common alloying elements in biodegradable implant materials. Conventional in vitro testing using heart, liver, and endothelial cell lines performed in parallel with zebrafish studies revealed statistically significant differences in toxicity (up to 100-fold), along with distinct changes in the morphology of the heart, liver, and blood vessels that were undetectable in cell cultures. These results indicate that our zebrafish model is a useful alternative to mammalian systems for accurately and rapidly evaluating the in vivo toxicity of newly developed metallic materials.
URI
http://pubs.kist.re.kr/handle/201004/68216
ISSN
2045-2322
Appears in Collections:
KIST Publication > Article
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