Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells
- Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells
- 이상희; 조아라; 성준; 남현성; 이효원; 박종민; 김환명; 김은하; 박승범
- Issue Date
- Bioconjugate chemistry
- VOL 29, NO 10-3401
- Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.
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