Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells

Title
Near-IR Fluorescent Tracer for Glucose-Uptake Monitoring in Live Cells
Authors
이상희조아라성준남현성이효원박종민김환명김은하박승범
Issue Date
2018-10
Publisher
Bioconjugate chemistry
Citation
VOL 29, NO 10-3401
Abstract
Fluorescent tracers for glucose-uptake monitoring could be used as chemical tools for diagnosis and for discovery of novel therapeutic agents via the development of phenotypic screening systems. Here we present a new near-infrared fluorescent glucose tracer, Glc-SiR-CO2H, for monitoring the cellular glucose uptake. By conjugating glucosamine with two different silicon rhodamine fluorochromes, we found that the net charge of fluorochromes has considerable effects on cellular uptake of the probe. Competition assay with d/l-glucose as well as Western blot analysis implied GLUT-dependent uptake mechanism of this probe. Finally, Glc-SiR-CO2H not only differentiates cancer cells from normal cells, but also allows monitoring anticancer effects in live cells.
URI
http://pubs.kist.re.kr/handle/201004/68888
ISSN
1043-1802
Appears in Collections:
KIST Publication > Article
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