Quantitative Proteome Analysis of Brain Subregions and Spinal Cord from Experimental Autoimmune Encephalomyelitis Mice by TMT-Based Mass Spectrometry

Title
Quantitative Proteome Analysis of Brain Subregions and Spinal Cord from Experimental Autoimmune Encephalomyelitis Mice by TMT-Based Mass Spectrometry
Authors
권오승민호필마흐법하산안카 라루카라하만김혜윤한도현
Keywords
proteomics; proteome map; eae; biomarker
Issue Date
2019-03
Publisher
Proteomics
Citation
VOL 19, NO 5-1800355-11
Abstract
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS); its cause is unknown. To understand the pathogenesis of MS, researchers often use the experimental autoimmune encephalomyelitis (EAE) mouse model. Here, our aim was to build a proteome map of the biological changes that occur during MS at the major onset sites-the brain and the spinal cord. We performed quantitative proteome profiling in five specific brain regions and the spinal cord of EAE and healthy mice with high-resolution mass spectrometry based on tandem mass tags. On average, we quantified 7,400 proteins per region, with the most differentially expressed proteins in the spinal cord (1,691), hippocampus (104), frontal cortex (83), cerebellum (63), brainstem (50), and caudate nucleus (41). Moreover, we identified region-specific and commonly expressed proteins in each region and performed bioinformatics analysis. Pathway analysis revealed that protein clusters resemble their functions in disease pathogenesis (i.e., by inducing inflammatory responses, immune activation, and cell-cell adhesion). In conclusion, our study provides an understanding of the pathogenesis of MS in the EAE animal model. We expect that our comprehensive proteome map of the brain and spinal cord can be used to identify biomarkers for the pathogenesis of MS. This article is protected by copyright.
URI
http://pubs.kist.re.kr/handle/201004/69068
ISSN
1615-9853
Appears in Collections:
KIST Publication > Article
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