Expanding the “minimalist” small molecule tagging approach to different bioactive compounds

Title
Expanding the “minimalist” small molecule tagging approach to different bioactive compounds
Authors
이준석Wenjie LangChaonan YuanBiwei ZhuSijun PanJian LiuJie LuoShikun NieQing ZhuJingyan Ge
Issue Date
2019-03
Publisher
Organic & biomolecular chemistry
Citation
VOL 17, NO 11-3017
Abstract
“Minimalist” small molecule tagging (MSMT) is a promising approach that easily converts bioactive compounds into affinity-based probes (AfBPs) for proteomic studies. In this work, seven bioactive compounds targeting diversified protein classes were installed with “minimalist” linkers through common reactions to generate the corresponding AfBPs. These probes were evaluated for cell-based protein profiling and target validation. Among them, the entinostat-derived probe EN and the camptothecin-derived probe CA were further utilized in cellular imaging and SILAC-based large-scale target identification. Our extensive studies suggest that the “minimalist” small molecule tagging approach could be expanded to different classes of bioactive compounds for modification into AfBPs as a dual functional tool for both proteomics and cellular imaging.
URI
http://pubs.kist.re.kr/handle/201004/69154
ISSN
1477-0520
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KIST Publication > Article
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