Differential Microbicidal Effects of Bimetallic Iron-Copper Nanoparticles on Escherichia coli and MS2 Coliphage

Title
Differential Microbicidal Effects of Bimetallic Iron-Copper Nanoparticles on Escherichia coli and MS2 Coliphage
Authors
홍석원김형은Hye-Jin LeeMin Sik KimTaewan KimHongshin LeeHak-Hyeon KimMin ChoChangha Lee
Keywords
Bimetallic iron?copper nanoparticles; Escherichia coli; MS2 coliphage; Microbicidal effects; oxidative damages
Issue Date
2019-03
Publisher
Environmental science & technology
Citation
VOL 53, NO 5-2687
Abstract
Bimetallic iron-copper nanoparticles (Fe/Cu-NPs) were synthesized by a single-pot surfactant-free method in aqueous solution [via the reduction of ferrous ion to zerovalent iron nanoparticles (Fe-NPs) and the subsequent copper-coating by metal ion exchange]. The produced Fe/Cu-NPs formed aggregates of spherical nanoparticles (approximately 30-70 nm) of Fe-Cu core-shell structures with 11 wt % copper content. The microbicidal effects of Fe/Cu-NPs were explored on Escherichia coli and MS2 coliphage, surrogates for bacterial and viral pathogens, respectively. Fe/Cu-NPs exhibited synergistically enhanced activity for the inactivation of E. coli and MS2, compared to single-metal nanoparticles (i.e., Fe-NPs and Cu-NPs). Various experiments (microbial inactivation tests under different conditions, fluorescence staining assays, experiments using ELISA and qRT-PCR, etc.) suggested that Fe/Cu-NPs inactivate E. coli and MS2 via dual microbicidal mechanisms. Two biocidal copper species [Cu(I) and Cu(III)] can be generated by different redox reactions of Fe/Cu-NPs. It is suggested that E. coli is strongly influenced by the cytotoxicity of Cu(I), while MS2 is inactivated mainly due to the oxidative damages of protein capsid and RNA by Cu(III).
URI
http://pubs.kist.re.kr/handle/201004/69209
ISSN
0013-936X
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