Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system

Title
Procaspase activating compound 1 controls tetracycline repressor-regulated gene expression system
Authors
심태보송치만이지연김남경박미리오기봉
Keywords
anti-induction; antagonist; gene regulation; PAC-1; tetracycline repressor; TetR-regulated system
Issue Date
2019-01
Publisher
Bioscience reports
Citation
VOL 39, NO 1, BSR20180793
Abstract
The tetracycline repressor (TetR)-regulated system is a widely used tool to study gene functions through control of its expression. Various effectors such as tetracycline (Tc) and doxycycline (Dox) quickly induce or shut down gene expression, but reversing gene expression has not been eligible due to long half-lives of such effectors. Here, we found that procaspase activating compound 1 (PAC-1) rapidly reduces transient expression of TetR-regulated green fluorescent protein (GFP) in mammalian cells. Next, we applied PAC-1 to control of expression of transient receptor potential melastatin 7 (TRPM7) protein, whose downstream cellular events can be monitored by cell morphological changes. We observed that PAC-1 quickly reduces TRPM7 expression, consequently affecting cell morphology regulated by TRPM7. The present study demonstrates the first small molecule that efficiently turns off the TetR-regulated gene expression in mammalian cells, thereby precisely regulating the expression level of target gene.
URI
http://pubs.kist.re.kr/handle/201004/69355
ISSN
0144-8463
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE