Reduction of Iridium Loading to the Minimum Level Required for Water Oxidation Electrocatalysis without Sacrificing the Electrochemical Stability

Title
Reduction of Iridium Loading to the Minimum Level Required for Water Oxidation Electrocatalysis without Sacrificing the Electrochemical Stability
Authors
김상훈쭝 히에우 쩐이재영장한샘
Issue Date
2019-05
Publisher
The Journal of Physical Chemistry C
Citation
VOL 123, NO 23-12934
Abstract
5-HT6 receptor (5-HT6R) is implicated in cognitive dysfunction, mood disorder, psychosis, and eating disorders. However, despite its significant role in regulating the brain functions, regulation of 5-HT6R at the molecular level is poorly understood. Here, using yeast two-hybrid assay, we found that human 5-HT6R directly binds to neuro-oncological ventral antigen 1 (Nova-1), a brain-enriched splicing regulator. The interaction between 5-HT6R and Nova-1 was confirmed using GST pull-down and co-immunoprecipitation assays in cell lines and rat brain. The splicing activity of Nova-1 was decreased upon overexpression of 5-HT6R, which was examined by detecting the spliced intermediates of gonadotropin-releasing hormone (GnRH), a known pre-mRNA target of Nova-1, using RT-PCR. In addition, overexpression of 5-HT6R induced the translocation of Nova-1 from the nucleus to cytoplasm, resulting in the reduced splicing activity of Nova-1. In contrast, overexpression of Nova-1 reduced the activity and the total protein levels of 5-HT6R. Taken together, these results indicate that when the expression levels of 5-HT6R or Nova-1 protein are not properly regulated, it may also deteriorate the function of the other.
URI
http://pubs.kist.re.kr/handle/201004/69470
ISSN
1932-7447
Appears in Collections:
KIST Publication > Article
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