ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain

Title
ANO1/TMEM16A regulates process maturation in radial glial cells in the developing brain
Authors
오수진황은미홍규상오우택김형섭최재혁이성훈이병준장용우정주영김인범
Keywords
Anoctamin 1; TMEM16A; neural stem cell; radial glial cell; cortical development
Issue Date
2019-06
Publisher
Proceedings of the National Academy of Sciences of the United States of America
Citation
VOL 116, NO 25-12499
Abstract
Neural stem cells (NSCs) are primary progenitor cells in the early developmental stage in the brain that initiate a diverse lineage of differentiated neurons and glia. Radial glial cells (RGCs), a type of neural stem cell in the ventricular zone, are essential for nurturing and delivering new immature neurons to the appropriate cortical target layers. Here we report that Anoctamin 1 (ANO1)/TMEM16A, a Ca2+-activated chloride channel, mediates the Ca2+-dependent process extension of RGCs. ANO1 is highly expressed and functionally active in RGCs of the mouse embryonic ventricular zone. Knockdown of ANO1 suppresses RGC process extension and protrusions, whereas ANO1 overexpression stimulates process extension. Among various trophic factors, brain-derived neurotrophic factor (BDNF) activates ANO1, which is required for BDNF-induced process extension in RGCs. More importantly, Ano1-deficient mice exhibited disrupted cortical layers and reduced cortical thickness. We thus conclude that the regulation of RGC process extension by ANO1 contributes to the normal formation of mouse embryonic brain.
URI
http://pubs.kist.re.kr/handle/201004/69543
ISSN
0027-8424
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KIST Publication > Article
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