Gold­nanoparticle­coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry

Title
Gold­nanoparticle­coated magnetic beads for concentration and ionization of analytes for laser desorption/ionization mass spectrometry
Authors
강민정김문주박종민노주윤윤태경변재철
Keywords
LDI-MS; gold-nanoparticle-coated magnetic beads; sample preparation; concentration
Issue Date
2019-03
Publisher
Rapid communications in mass spectrometry : RCM
Citation
VOL 33, NO 5-538
Abstract
Rationale Magnetic particles coated with gold nanoparticles (Au NPs) (Au‐ MAGs) were developed and used (1) for sample concentration and (2) as a solid matrix for laser adsorption/desorption mass spectrometry (LDI‐ MS). Methods The Au‐ MAGs were prepared by (1) coating polystyrene on iron oxide NPs (PS‐ MNPs), (2) coating poly‐ l‐ lysine on the PS‐ MNPs (PLL‐ coated PS‐ MNPs), and (3) coating negatively charged Au NPs on the PLL‐ MNPs (Au‐ MAGs). Results The Au‐ MAGs were used to concentrate the target analyte by means of electrostatic interactions between the positively charged GHP9 and the negatively charged Au‐ MAGs as well as selective interactions (such as gold– sulfur interactions) between glutathione (GSH) and Au‐ MAGs. Then, the concentrated analyte could be ionized for LDI‐ MS. Conclusions The Au‐ MAGs were demonstrated (1) to concentrate the target analyte in a sample solution, tested by electrostatic interactions and selective interactions between gold and sulfur and (2) to ionize the concentrated analyte for LDI‐ MS.
URI
http://pubs.kist.re.kr/handle/201004/69574
ISSN
0951-4198
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KIST Publication > Article
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