Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic beta-cell Metabolism

Title
Hypoxylonol F Isolated from Annulohypoxylon annulatum Improves Insulin Secretion by Regulating Pancreatic beta-cell Metabolism
Authors
함정엽김태정최필주송봉근김영석이다혜황병수Noriko Yamabe황기서강기성
Keywords
Annulohypoxylon annulatum; insulin; PI3K; Akt; PPAR gamma; PDX-1
Issue Date
2019-08
Publisher
Biomolecules
Citation
VOL 9-335-10
Abstract
Insulin plays a key role in glucose homeostasis and is hence used to treat hyperglycemia, the main characteristic of diabetes mellitus. Annulohypoxylon annulatum is an inedible ball-shaped wood-rotting fungus, and hypoxylon F is one of the major compounds of A. annulatum. The aim of this study is to evaluate the e ects of hypoxylonol F isolated from A. annulatum on insulin secretion in INS-1 pancreatic -cells and demonstrate the molecular mechanisms involved. Glucose-stimulated insulin secretion (GSIS) values were evaluated using a rat insulin ELISA kit. Moreover, the expression of proteins related to pancreatic -cell metabolism and insulin secretion was evaluated using Western blotting. Hypoxylonol F isolated from A. annulatum was found to significantly enhance glucose-stimulated insulin secretion without inducing cytotoxicity. Additionally, hypoxylonol F enhanced insulin receptor substrate-2 (IRS-2) levels and activated the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway. Interestingly, it also modulated the expression of peroxisome proliferator-activated receptor (PPAR) and pancreatic and duodenal homeobox 1 (PDX-1). Our findings showed that A. annulatum and its bioactive compounds are capable of improving insulin secretion by pancreatic -cells. This suggests that A. annulatum can be used as a therapeutic agent to treat diabetes.
URI
http://pubs.kist.re.kr/handle/201004/69731
ISSN
2218-273X
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KIST Publication > Article
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