DNA skybridge: 3D structure producing a light sheet for high-throughput single-molecule imaging

Title
DNA skybridge: 3D structure producing a light sheet for high-throughput single-molecule imaging
Authors
정철현김대형Fahad Rashid조연모Manal S. Zaher조일환Samir M. Hamdan이종봉
Keywords
DNA; Semiconductor process; Protein Diffusion; Single molecule imaging
Issue Date
2019-10
Publisher
Nucleic acids research
Citation
VOL 47, NO 18-e107-8
Abstract
Real-time visualization of single-proteins or - complexes on nucleic acid substrates is an essential tool for characterizing nucleic acid binding proteins. Here, we present a novel surface-condition independent and high-throughput single-molecule optical imaging platform called ‘DNA skybridge’. The DNA skybridge is constructed in a 3D structure with 4 m-high thin quartz barriers in a quartz slide. Each DNA end is attached to the top of the adjacent barrier, resulting in the extension and immobilization of DNA. In this 3D structure, the bottom surface is outof- focus when the target molecules on the DNA are imaged. Moreover, the DNA skybridge itself creates a thin Gaussian light sheet beam parallel to the immobilized DNA. This dual property allows for imaging a single probe-tagged molecule moving on DNA while effectively suppressing interference with the surface and background signals from the surface.
URI
http://pubs.kist.re.kr/handle/201004/70017
ISSN
0305-1048
Appears in Collections:
KIST Publication > Article
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