Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome

Title
Common Repository of FBS Proteins (cRFP) To Be Added to a Search Database for Mass Spectrometric Analysis of Cell Secretome
Authors
이철주이지은안영희양원석박예은신지혜백지현이수진카비르플라산트홍은영염정훈정현교안희성이선정주신영권유미신성호나승진최선영김은희박성준방오영하철원이진원강운범김혜정박강식이진환김진영김광표김영수Hisashi HiranoEugene C. Yi조제열백은옥
Issue Date
2019-10
Publisher
Journal of proteome research
Citation
VOL 18, NO 10-3806
Abstract
We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search of cell secretomes. cRFP is a small supplementary sequence list of highly abundant fetal bovine serum proteins added to the reference database in use. The aim behind using cRFP is to prevent the contaminant FBS proteins from being misidentified as other proteins in the reference database, just as we would use cRAP (common Repository of Adventitious Proteins) to prevent contaminant proteins present either by accident or through unavoidable contacts from being misidentified as other proteins. We expect it to be widely used in experiments where the proteins are obtained from serum-free media after thorough washing of the cells, or from a complex media such as SILAC, or from extracellular vesicles directly.
URI
http://pubs.kist.re.kr/handle/201004/70049
ISSN
1535-3893
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