Kinetics of Lipid Raft Formation at Lipid Monolayer-Bilayer Junction Probed by Surface Plasmon Resonance

Title
Kinetics of Lipid Raft Formation at Lipid Monolayer-Bilayer Junction Probed by Surface Plasmon Resonance
Authors
유용상Hansik YunTaerin ChungJeng-Hun SuhSungho KimKyookeun LeeNathan J. WittenbergSang-Hyun OhByoungho LeeSin-Doo Lee
Keywords
Lipid raft; Lipid monolayer-bilayer junction; Real-time; Surface plasmon resonance; Optical sensors; Lipid-lipid interaction
Issue Date
2019-10
Publisher
Biosensors and bioelectronics
Citation
VOL 142-111568-8
Abstract
A label-free, non-dispruptive, and real-time analytical device to monitor the dynamic features of biomolecules and their interactions with neighboring molecules is an essential prerequisite for biochip- and diagonostic assays. To explore one of the central questions on the lipid-lipid interactions in the course of the liquid-ordered (lo) domain formation, called rafts, we developed a method of reconstituting continuous but spatially heterogeneous lipid membrane platforms with molayer-bilayer juntions (MBJs) that enable to form the lo domains in a spatiotemporally controlled manner. This allows us to detect the time-lapse dynamics of the lipid-lipid interactions during raft formation and resultant membrane phase changes together with the raft-associated receptor-ligand binding through the surface plasmon resonance (SPR). For cross-validation, using epifluorescence microscopy, we demonstrated the underlying mechanisms for raft formations that the infiltration of cholesterols into the sphingolipid-enriched domains plays a crucial roles in the membrane phase-separation. Our membrane platform, being capable of monitoring dynamic interactions among lipids and performing the systematic optical analysis, will unveil physiological roles of cholesterols in a variety of biological events.
URI
http://pubs.kist.re.kr/handle/201004/70095
ISSN
0956-5663
Appears in Collections:
KIST Publication > Article
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