Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity

Title
Chimeric crRNAs with 19 DNA residues in the guide region show the retained DNA cleavage activity of Cas9 with potential to improve the specificity
Authors
안대로정학숙정철현강성재박지현장정은이희재김효영전용문안진수안종성방두희
Keywords
CRISPR; Cas9; Chimeric crRNA; target specificity
Issue Date
2019-03
Publisher
Chemical communications
Citation
VOL 55, NO 24-3555
Abstract
We demonstrated that 19 out of 20 RNA residues in the guide region of crRNA can be replaced with DNA residues with high GC-contents. The cellular activity of the chimeric crRNAs to disrupt the target gene was comparable to that of the native crRNA.
URI
http://pubs.kist.re.kr/handle/201004/70175
ISSN
1359-7345
Appears in Collections:
KIST Publication > Article
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