Hypersensitive antibiotic susceptibility test based on a beta-lactamase assay with a parylene-matrix chip
- Hypersensitive antibiotic susceptibility test based on a beta-lactamase assay with a parylene-matrix chip
- 강민정; 박종민; 김조일; 노주윤; 김미라; 변재철
- Laser desorption/ionization time-of-flight; LDI-TOF MS; Parylene-matrix chip; Antibiotic susceptibility test; Beta-lactamase
- Issue Date
- Enzyme and microbial technology
- VOL 97-96
- Many kinds of susceptibility test for β-lactam antibiotics have been used to determine the antibiotic resistance of bacterial strains. Here, a sensitive antibiotic susceptibility test was presented by using a specialized reaction tool for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) based on parylene-matrix chip. The β-lactamase assay was carried out in a specialized reaction tool by (1) concentrating the bacterial strain and (2) incubating the bacteria with penicillin-G. The parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array, and the products of β-lactamase reaction in the low range of mass-to-charge ratio (m/z < 500) could be effectively analyzed by using a parylene-matrix chip. The sensing parameters were compared with conventional chromogenic antibiotic susceptibility test for β-lactam antibiotics. Finally, LDI-TOF MS with a specialized reaction tool and parylene-matrix chip could achieve a limit of detection as low as 600 cells/spot for penicillin-G.
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