Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics

Title
Fluorescent protein voltage probes derived from ArcLight that respond to membrane voltage changes with fast kinetics
Authors
브래들리 베이커래리코헨Zhou HanLei JinJelena PlatisaVincent A. Pieribone
Issue Date
2013-11
Publisher
PLoS ONE
Citation
VOL 8, NO 11-e81295-9
Abstract
We previously reported the discovery of a fluorescent protein voltage probe, ArcLight, and its derivatives that exhibit large changes in fluorescence intensity in response to changes of plasma membrane voltage. ArcLight allows the reliable detection of single action potentials and sub-threshold activities in individual neurons and dendrites. The response kinetics of ArcLight (τ1-on ~10 ms, τ2-on ~ 50 ms) are comparable with most published geneticallyencoded voltage probes. However, probes using voltage-sensing domains other than that from the Ciona intestinalis voltage sensitive phosphatase exhibit faster kinetics. Here we report new versions of ArcLight, in which the Ciona voltage-sensing domain was replaced with those from chicken, zebrafish, frog, mouse or human. We found that the chicken and zebrafish-based ArcLight exhibit faster kinetics, with a time constant (τ) less than 6ms for a 100 mV depolarization. Although the response amplitude of these two probes (8-9%) is not as large as the Ciona-based ArcLight (~35%), they are better at reporting action potentials from cultured neurons at higher frequency. In contrast, probes based on frog, mouse and human voltage sensing domains were either slower than the Ciona-based ArcLight or had very small signals.
URI
http://pubs.kist.re.kr/handle/201004/70426
ISSN
1932-6203
Appears in Collections:
KIST Publication > Article
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