Optimization of structured illumination microscopy with designing and rotating a grid pattern using a spatial light modulator

Title
Optimization of structured illumination microscopy with designing and rotating a grid pattern using a spatial light modulator
Authors
박민철유낙원강지훈한정헌Byeong Kwon Ju
Issue Date
2019-09
Publisher
Optical engineering
Citation
VOL 58, NO 9-094102-8
Abstract
Our structured illumination microscopy (SIM) is based on a spatial light modulator (SLM) instead of an illumination mask, which does not need to be attached to a linear stage. This SIM can easily design the period of the one-dimensional grid related to the optical sectioning strength and can rapidly acquire three-dimensional data. The optimization of SIM with an SLM is proposed. Previous studies primarily varied magnification with a high numerical aperture objective to optimize the axial response. It is feasible to obtain the maximum optical sectioning strength by designing a grid pattern that has an appropriately high spatial frequency and to uniformly cover the entire frequency spectrum of the sample by rotating a grid pattern. We have successfully optimized SIM with such a grid and covered the frequency spectrum by rotating a grid pattern in multiple orientations.
URI
http://pubs.kist.re.kr/handle/201004/70479
ISSN
0091-3286
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE