Full metadata record

DC FieldValueLanguage
dc.contributor.author황은미-
dc.contributor.author정현국-
dc.contributor.author김승찬-
dc.contributor.author김영은-
dc.contributor.author김아정-
dc.contributor.author박재용-
dc.contributor.author이석근-
dc.date.accessioned2021-06-09T04:23:30Z-
dc.date.available2021-06-09T04:23:30Z-
dc.date.issued2019-11-
dc.identifier.citationVOL 20-5902-15-
dc.identifier.issn1422-0067-
dc.identifier.other53935-
dc.identifier.urihttp://pubs.kist.re.kr/handle/201004/70545-
dc.description.abstractTWIK-related potassium channel-1 (TREK-1) is broadly expressed in the brain and involved in diverse brain diseases, such as seizures, ischemia, and depression. However, the cell type-specific roles of TREK-1 in the brain are largely unknown. Here, we generated a Cre-dependent TREK-1 knockdown (Cd-TREK-1 KD) transgenic mouse containing a gene cassette for Cre-dependent TREK-1 short hairpin ribonucleic acid to regulate the cell type-specific TREK-1 expression. We confirmed the knockdown of TREK-1 by injecting adeno-associated virus (AAV) expressing Cre into the hippocampus of the mice. To study the role of hippocampal neuronal TREK-1 in a lipopolysaccharide (LPS)-induced depression model, we injectedAAV-hSyn-BFP (nCTL group) orAAV-hSyn-BFP-Cre (nCre group) virus into the hippocampus of Cd-TREK-1 KD mice. Interestingly, the immobility in the tail suspension test after LPS treatment did not change in the nCre group. Additionally, some neurotrophic factors (BDNF, VEGF, and IGF-1) significantly increased more in the nCre group compared to the nCTL group after LPS treatment, but there was no di erence in the expression of their receptors. Therefore, our data suggest that TREK-1 in the hippocampal neurons has antidepressant e ects, and that Cd-TREK-1 KD mice are a valuable tool to reveal the cell type-specific roles of TREK-1 in the brain.-
dc.publisherInternational journal of molecular sciences-
dc.titleThe Knockdown of TREK-1 in Hippocampal Neurons Attenuate Lipopolysaccharide-Induced Depressive-Like Behavior in Mice-
dc.typeArticle-
dc.relation.page5902-15902-15-
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE