Affinity-based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors
- Affinity-based Protein Profiling Reveals Cellular Targets of Photoreactive Anticancer Inhibitors
- 이준석; Nan Ma; Zhi-Min Zhang; Ke Cheng; Legen Lin; Dong-Mei Zhang; Piliang Hao; Ke Ding; Wen-Cai Ye; Zhengqiu Li
- affinity profiling; drug; photo-crosslinking
- Issue Date
- ACS Chemical Biology
- VOL 14, NO 12-2552
- Affinity-based protein profiling has proven to be a powerful method in target identification of bioactive molecules. Here, this technology was applied in two photoreactive anticancer inhibitors, arenobufagin and HM30181. Using UV irradiation, these photoreactive reagents can covalently cross-link to target proteins, leading to a covalent binding with target proteins. Moreover, the cellular on/off targets of these two molecules, including ATP1A1, MDR1, PARP1, DDX5, NOP2, RAB6A, and ERGIC1 were first identified by affinity-based protein profiling and bioimaging approaches. The protein hit, PARP1, was further validated to be involved in the function of the anticancer effects.
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