Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins

Title
Highly sensitive three-dimensional interdigitated microelectrode biosensors embedded with porosity tunable hydrogel for detecting proteins
Authors
최낙원최웅선김혜진김진식최정규황교선
Issue Date
2020-01
Publisher
Sensors and actuators. B, Chemical
Citation
VOL 302-127190-17
Abstract
This study focused on fabricating a target-specific electrical biosensor with high sensitivity and dynamic range by embedding a hydrogel, that incorporated a much larger number of antibodies in the reaction region of the sensor, allowing all biomolecules except the target molecules to be filtered off. The biosensor is comprised of a three-dimensional interdigitated microelectrode (3D IMEs) with much higher aspect ratios than conventional IMEs and a hydrogel with a 3D mesh structure. The hydrogel increases the number of antibodies in the reaction region of the 3D IMEs by approximately 1,000-fold and enhances the impedance change induced by the specific binding of biomolecules. The hydrogel density is altered according to the target biomolecules. The impedance change by Aβ42 binding was maximized to approximately 12.135  ±  0.903% in the 3D IMEs with a ‘dense’ hydrogel. The change by prostate-specific antigen (PSA) binding was maximized in the 3D IMEs with a ‘loose’ hydrogel. Thus, the dynamic range and sensitivity for detecting Aβ42 were enhanced by a maximum of two orders and by approximately 2.58-fold compared to planar IMEs. These results demonstrate the high performance of the hydrogel-embedded 3D IMEs and its applicability for biological fluid-based diagnosis.
URI
http://pubs.kist.re.kr/handle/201004/70675
ISSN
0925-4005
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE