sigma 1 receptors activate astrocytes via p38 MAPK phosphorylation leading to the development of mechanical allodynia in a mouse model of neuropathic pain
- sigma 1 receptors activate astrocytes via p38 MAPK phosphorylation leading to the development of mechanical allodynia in a mouse model of neuropathic pain
- 최훈성; 윤서연; Ji-Young Moon; Dae-Hyun Roh; Sheu-Ran Choi; Soon-Gu Kwon; Suk-Yun Kang; Ho-Jae Han; Alvin J. Beitz; Seog-Bae Oh; Jang-Hern Lee
- Issue Date
- British journal of pharmacology
- VOL 171, NO 24-5897
- Background and PurposeSpinal astrocytes have emerged as important mechanistic contributors to the genesis of mechanical allodynia (MA) in neuropathic pain. We recently demonstrated that the spinal sigma non-opioid intracellular receptor 1 (sigma 1 receptor) modulates p38 MAPK phosphorylation (p-p38), which plays a critical role in the induction of MA in neuropathic rats. However, the histological and physiological relationships among sigma 1, p-p38 and astrocyte activation is unclear.
Experimental ApproachWe investigated: (i) the precise location of sigma 1 receptors and p-p38 in spinal dorsal horn; (ii) whether the inhibition of sigma 1 receptors or p38 modulates chronic constriction injury (CCI)-induced astrocyte activation; and (iii) whether this modulation of astrocyte activity is associated with MA development in CCI mice.
Key ResultsThe expression of sigma 1 receptors was significantly increased in astrocytes on day 3 following CCI surgery. Sustained intrathecal treatment with the sigma 1 antagonist, BD-1047, attenuated CCI-induced increase in GFAP-immunoreactive astrocytes, and the treatment combined with fluorocitrate, an astrocyte metabolic inhibitor, synergistically reduced the development of MA, but not thermal hyperalgesia. The number of p-p38-ir astrocytes and neurons, but not microglia was significantly increased. Interestingly, intrathecal BD-1047 attenuated the expression of p-p38 selectively in astrocytes but not in neurons. Moreover, intrathecal treatment with a p38 inhibitor attenuated the GFAP expression, and this treatment combined with fluorocitrate synergistically blocked the induction of MA.
Conclusions and ImplicationsSpinal sigma 1 receptors are localized in astrocytes and blockade of sigma 1 receptors inhibits the pathological activation of astrocytes via modulation of p-p38, which ultimately prevents the development of MA in neuropathic mice.
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