Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method

Title
Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method
Authors
김병찬김혜리송민영
Keywords
Centrifugation-based partitioning; Rapid isolation; Bacteria-speci?c aptamer; Non-SELEX-Based method; Escherichia coli
Issue Date
2020-02
Publisher
Analytical biochemistry
Citation
VOL 591, 113542
Abstract
Usually, isolation of bacteria-specifi c aptamers by SELEX is a time-consuming process due to the required repeated rounds of binding, separation, and amplifi cation of the probes to target bacteria. Here, we show that it is possible to isolate bacteria-specifi c DNA aptamers omitting the repeated rounds of binding incubation, separation, and amplifi cation that are indispensable for SELEX. The serial removal of unbound DNAs to the bacterial cells from an initial mixture of bacteria and DNA libraries through serial centrifugation, one-time separation, and further one-time amplifi cation of DNA bound to the target bacterial cells applied in this nonSELEX-based method allows successful aptamer isolation.
URI
http://pubs.kist.re.kr/handle/201004/70952
ISSN
0003-2697
Appears in Collections:
KIST Publication > Article
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