Continuous Surveillance of Bioaerosols On-Site Using an Automated Bioaerosol-Monitoring System

Continuous Surveillance of Bioaerosols On-Site Using an Automated Bioaerosol-Monitoring System
indoor air quality; automated bioaerosol-monitoring system; bioaerosols; continuous surveillance; wet-cyclone; luciferase/luciferin immobilization
Issue Date
ACS sensors
VOL 5, NO 2-405
Real-time on-site monitoring of bioaerosols in an air environment is important for preventing various adverse health eff ects including respiratory diseases and allergies caused by bioaerosols. Here, we report the development of an on-site automated bioaerosol-monitoring system (ABMS) using integrated units including a wet-cyclone bioaerosol sampler, a thermal-lysis unit for extracting adenosine triphosphate (ATP), an ATPdetection unit based on the immobilization of luciferase/luciferin for bioluminescence reactions, and a photomultiplier tube-based detector. The performance of the bioaerosol detection system was verifi ed using Escherichia coli (E. coli) as a model source. Each unit was optimized to process ∼9.6 × 105 times the concentrated ratio of collected bioaerosol samples, using a 3 min lysis time to extract ATP, and has a detection limit of ∼375 colony-forming units (CFUs)/mL with more than 30 days of stability for the immobilizedluciferase/luciferin detection unit supported by a glass-fi ber conjugation pad. After the integration of all units, the ABMS achieved E. coli bioaerosol monitoring with continuous detection at 5 min intervals and a minimum detection limit of ∼130 CFU/mair3. Furthermore, the rapid responsivity and stable operation performance of the ABMS under test-bed conditions and during a fi eld test demonstrated that the ABMS is capable of continuously monitoring bioaerosols in real-time with high sensitivity. The monitoring system developed here with immobilization strategies for bioluminescence reactions triggered by ATP extracted from collected bioaerosol samples using a simple heat-lysis method may help establish sustainable platforms to obtain stable signals for the real-time detection of bioaerosols on-site.
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