Interaction between marine bacterium Stappia sp. K01 and diatom Phaeodactylum tricornutum through extracellular fatty acids

Title
Interaction between marine bacterium Stappia sp. K01 and diatom Phaeodactylum tricornutum through extracellular fatty acids
Authors
김상민Trang Thi Vuong권벼리엄재인신복규
Keywords
extracellular fatty acid; Phaeodactylum tricornutum; Stappia sp. K01; Microalgae-bacteria interaction; fucoxanthin
Issue Date
2020-02
Publisher
Journal of applied phycology
Citation
VOL 32-82
Abstract
Fucoxanthin, a marine carotenoid with biological activities including anti-obesity activity, is generally produced from macroalgae, but several microalgae have been suggested as new sources. A standardized fucoxanthin extract was recently developed from the diatom Phaeodactylum tricornutum. Here, we illustrate that a marine bacterium, Stappia sp. K01, isolated from xenic cultures of P. tricornutum, improves growth (72%) and pigment biosynthesis (172% fucoxanthin and 144% chlorophylls, respectively) of P. tricornutum by co-culture. In addition, sixmajor extracellular fatty acids (FAs) including C14:0, C16:0, C18:0, C16:1, C18:1, and C20:5 secreted by P. tricornutum play an important role in this effect of Stappia sp. K01. These FAs were secreted from P. tricornutum in a range of 0.1~4.9 μg L− 1 and each maximum concentration in the culture medium was decreased to 25~83% by Stappia sp. K01 inoculation. In the treatment of a mixed FA solution with their maximum concentrations to P. tricornutum culture, these FAs showed 30% growth inhibition of P. tricornutum and this effect was removed by Stappia sp. K01 inoculation. Each FA was utilized as a nutrient for Stappia sp. K01 in M9 minimum medium. Several growth regulation-related genes in P. tricornutum were downregulated by FA treatment and upregulated by co-culturing with Stappia sp. K01. These results indicate that Stappia sp. K01 can be used as an inducer to promote biomass production and fucoxanthin biosynthesis in P. tricornutum.
URI
http://pubs.kist.re.kr/handle/201004/71147
ISSN
0921-8971
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