Obscurin and CLusterin Elecvation in Serum of Acute Myocardial Infarction Patients
- Obscurin and CLusterin Elecvation in Serum of Acute Myocardial Infarction Patients
- 강민정; 조예타 마흐무드; 성윤서; 누엔 탄 빈
- Moderate abundant proteins; Acute myocardial infarction biomarker candidates; Rapid screening; UHPLC-LTQ-Orbitrap; Obscurin and CLusterin
- Issue Date
- Bulletin of the Korean Chemical Society
- VOL 41, NO 3-273
- We discovered new biomarker candidates for acute myocardial infarction (AMI) using micro LC and accurate mass spectrometry. Two serum samples were pooled and used for biomarker screening. UHPLCLTQ-Orbitrap analysis was used with data-dependent acquisition followed by Sequest search. Six biomarker candidates were selected after fold change analysis of peptides in AMI patient samples compared to healthy controls. Literature search results were reflected. The six biomarker candidates were characterized and quantified using multi reaction monitoring using synthesized peptides or trypsin digested protein standards. Levels of obscurin, alpha-1-acid glycoprotein 1, and clusterin were significantly increased (p < 0.001) in AMI patient samples compared to levels in healthy controls, while those of antithrombin III and fibrinogen beta were not. We confirmed the diagnostic performance of obscurin and clusterin as biomarker candidates for AMI using commercial ELISA kits. Receiver operating characteristic results showed that obscurin and clusterin showed highest sensitivity and specificity for AMI diagnosis together with troponins by both of multi-reaction monitoring analysis and ELISA.
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