Suppression of TRPM7 enhances TRAIL­induced apoptosis in triple-negative breast cancer cells

Title
Suppression of TRPM7 enhances TRAIL­induced apoptosis in triple-negative breast cancer cells
Authors
송치만최승혜오기봉심태보
Keywords
TRAIL; TRPM7; apoptosis; c-FLIP; triple-negative breast cancer
Issue Date
2020-12
Publisher
Journal of cellular physiology
Citation
VOL 235, NO 12-10050
Abstract
Transient receptor potential cation channel subfamily M member 7 (TRPM7) composed of an ion channel and a kinase domain regulates triple‐ negative breast cancer (TNBC) cell migration, invasion, and metastasis, but it does not modulate TNBC proliferation. However, previous studies have shown that the combination treatment of nonselective TRPM7 channel inhibitors (2‐ aminoethoxydiphenyl borate and Gd3+) with tumor necrosis factor‐ related apoptosis‐ inducing ligand (TRAIL) increases antiproliferative effects and apoptosis in prostate cancer cells and hepatic stellate cells. We, therefore, investigated the potential role of TRPM7 in proliferation and apoptosis of TNBC cells (MDA‐ MB‐ 231 and MDA‐ 468 cells) with TRAIL. We demonstrated that suppression of TRPM7 via TRPM7 knockdown or pharmacological inhibition synergistically increases TRAIL‐ induced antiproliferative effects and apoptosis in TNBC cells. Furthermore, we showed that the synergistic interaction might be associated with TRPM7 channel activities using combination treatments of TRAIL and TRPM7 inhibitors (NS8593 as a TRPM7 channel inhibitor and TG100‐ 115 as a TRPM7 kinase inhibitor). We reveal that downregulation of cellular FLICE‐ inhibitory protein via inhibition of Ca2+ influx might be involved in the synergistic interaction. Our study would provide both a new role of TRPM7 in TNBC cell apoptosis and a potential combinatorial therapeutic strategy using TRPM7 inhibitors with TRAIL in the treatment of TNBC.
URI
http://pubs.kist.re.kr/handle/201004/71886
ISSN
0021-9541
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KIST Publication > Article
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