Optimization, validation, and comparison of a rapid method for the quantification of insulin­like growth factor 1 in serum using liquid chromatography-high-­resolution mass spectrometry

Title
Optimization, validation, and comparison of a rapid method for the quantification of insulin­like growth factor 1 in serum using liquid chromatography-high-­resolution mass spectrometry
Authors
권오승민호필성창민김민영박지수서윤담이화정
Keywords
Doping; IGF-1; growth hormone; hGH biomarker
Issue Date
2021-02
Publisher
DRUG TESTING AND ANALYSIS
Citation
VOL 13, NO 2-459
Abstract
Human insulin­like growth factor 1 (IGF­I) is the primary mediator of the effects of the growth hormone (GH). Therefore, it has been used as a biomarker to detect the abuse of GH in sports. The measurement of IGF­I relies on mass­based and immunological approaches to analysis. Among the mass­based analysis methods, liquid chromatography?mass spectrometry (LC?MS) has a number of functional advantages. LC?MS measurements based on the quantification of IGF­I, according to trypsin digestion, are used in the most common method of analyzing doping. However, this method is time­consuming and subject to experimental variability.In this study, we optimized a rapid method for detecting IGF­I without the trypsin digestion step. This method of analysis uses an ultra­centrifugal filter and an LC­HRMS through narrow­range mass scan method. To verify the validity of this method, eight categories of validation testing were applied with the following results: linearity, R2 > 0.99; limit of detection,
URI
http://pubs.kist.re.kr/handle/201004/71918
ISSN
1942-7603
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE