Femtosecond laser induced nano-textured micropatterning to regulate cell functions on implanted biomaterials

Title
Femtosecond laser induced nano-textured micropatterning to regulate cell functions on implanted biomaterials
Authors
석현광김유찬옥명렬전호정서현선서영민박재호김새로미이현수이경우전인동김영진유영식최병찬최종훈주천기
Issue Date
2020-10
Publisher
Acta Biomaterialia
Citation
VOL 116, NO 15-148
Abstract
Posterior capsular opacification (PCO) is the most common complication of cataract surgery. PCO is due to the proliferation, migration, and epithelial-to-mesenchymal transition of the residual lens epithelial cells (LECs) within the lens capsule. As surface topography influences cellular response, we investigated the effect of modulating the dimensions of periodic nano-textured patterns on the surface of an intraocular lens material to regulate lens epithelial cell functions such as cell adhesion, migration, orientation, and proliferation. Patterned poly(HEMA) samples were prepared by a femtosecond laser microfabrication, and the behaviors of human B-3 LECs were observed on groove/ridge patterns with widths varying from 5 to 40 µ m. In the presence of ridge and groove patterns, the adherent cells elongated along the direction of the patterns, and f-actin of the cells was spread to a lesser extent on the nano-textured groove surfaces. Both single and collective cell migrations were significantly inhibited in the perpendicular direction of the patterns on the nano-textured micro-patterned samples. We also fabricated the patterns on the curved surface of a commercially available intraocular lens for in vivo evaluation. In vivo results showed that a patterned IOL could help suppress the progression of PCO by inhibiting cell migration from the edge to the center of the IOL. Our reports demonstrate that nano- and microscale topographical patterns on a biomaterial surface can regulate cellular behavior when it is implanted into animals.
URI
http://pubs.kist.re.kr/handle/201004/71947
ISSN
1742-7061
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