Study and evaluation of the potential of lipid nanocarriers for transdermal delivery of siRNA

Title
Study and evaluation of the potential of lipid nanocarriers for transdermal delivery of siRNA
Authors
전호정이경우민대진최용현김지원윤세미장재희박수민타나카 마사요시조용우구형준최종훈
Keywords
1,2-dioleoyl-3-trimethylammonium-propane; gene delivery; lipofectamine; nanocarrier; siRNA; transdermal
Issue Date
2020-12
Publisher
BIOTECHNOLOGY JOURNAL
Citation
VOL 51, NO 12-6
Abstract
The topical delivery of siRNA‐ based therapies has opened new avenues for the treatment of skin disorders. The use of siRNA as a therapeutic, however, is limited due to its rapid degradation and poor cellular uptake. Furthermore, the top layer of skin, the stratum corneum, is a major barrier to the delivery of topical agents. There is an unmet need for efficient topical formulations for delivering siRNA to the site of action. In this study, 1,2‐ dioleoyl‐ 3‐ trimethylammonium‐ propane (DOTAP) or lipofectamine is used to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3‐ phosphate dehydrogenase (GAPDH); GAPDH expression is then evaluated at the cellular level. In addition, a dermal transport assay is designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier is significantly better than the delivery of siRNA without it. Thus, the findings identify lipid nanocarriers as excellent candidates for the transdermal delivery of siRNA for gene silencing in the skin and thus for applications in related preclinical models.
URI
http://pubs.kist.re.kr/handle/201004/72080
ISSN
1860-6768
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KIST Publication > Article
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