Study and evaluation of the potential of lipid nanocarriers for transdermal delivery of siRNA
- Study and evaluation of the potential of lipid nanocarriers for transdermal delivery of siRNA
- 전호정; 이경우; 민대진; 최용현; 김지원; 윤세미; 장재희; 박수민; 타나카 마사요시; 조용우; 구형준; 최종훈
- 1,2-dioleoyl-3-trimethylammonium-propane; gene delivery; lipofectamine; nanocarrier; siRNA; transdermal
- Issue Date
- BIOTECHNOLOGY JOURNAL
- VOL 51, NO 12-6
- The topical delivery of siRNA‐ based therapies has opened new avenues for the treatment of skin disorders. The use of siRNA as a therapeutic, however, is limited due to its rapid degradation and poor cellular uptake. Furthermore, the top layer of skin, the stratum corneum, is a major barrier to the delivery of topical agents. There is an unmet need for efficient topical formulations for delivering siRNA to the site of action. In this study, 1,2‐ dioleoyl‐ 3‐ trimethylammonium‐ propane (DOTAP) or lipofectamine is used to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3‐ phosphate dehydrogenase (GAPDH); GAPDH expression is then evaluated at the cellular level. In addition, a dermal transport assay is designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier is significantly better than the delivery of siRNA without it. Thus, the findings identify lipid nanocarriers as excellent candidates for the transdermal delivery of siRNA for gene silencing in the skin and thus for applications in related preclinical models.
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