Fabrication of glass micropipette device using reflow processes and its integration with microfluidic channels for patch clamp recording of cell

Title
Fabrication of glass micropipette device using reflow processes and its integration with microfluidic channels for patch clamp recording of cell
Authors
임매순윤영준남윤재이승기박재형
Keywords
Glass micropipette device; Microfluidic channels; Patch clamp; Gigaseal
Issue Date
2020-12
Publisher
IEEE sensors journal
Citation
VOL 20, NO 24-14702
Abstract
This paper presents a borosilicate glass micropipette device that has a high-aspect-ratio pore for patch clamp recording applications. The device was fabricated by carrying out glass reflow processes on a silicon substrate. The shape of the micropipette was determined by the width and thickness of the reflowed glass structure, which matches well with the rounded tip of a conventional glass pipette. The diameter of the micropipette pore was first defined by deep reactive ion etching (DRIE) of a silicon pillar, which was positioned at the center of the glass structure. Then, the silicon pillar was removed, and the pore diameter was additionally reduced to the submicron scale by depositing a parylene-C film. The fabricated device was integrated with microfluidic channels for patch clamp recording experiments: microfluidic PDMS layers were assembled on the top and bottom surfaces of the micropipette chip for the medium exchange and the application of negative pressure, respectively. The quality of seal between a cerebral cortex cell of a rat and the glass micropipette was characterized by the amplitude of the current produced by an applied voltage pulse. A negative pressure applied through the bottom microfluidic channel pulled down the cell and increased the seal resistance from 9.82 MΩ to 2.06 GΩ. This indicates that the gigaseal was successfully formed around the micropipette pore. In addition, the action potentials of the patched cortical neuron were recorded using the fabricated device.
URI
http://pubs.kist.re.kr/handle/201004/72112
ISSN
1530-437X
Appears in Collections:
KIST Publication > Article
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