Lung-targeted delivery of TGF-β antisense oligonucleotides to treat pulmonary fibrosis

Title
Lung-targeted delivery of TGF-β antisense oligonucleotides to treat pulmonary fibrosis
Authors
안대로김세훈강성재히엔 타이김정현이서경김경란전슬기이윤실
Issue Date
2020-06
Publisher
Journal of controlled release
Citation
VOL 322-121
Abstract
Pulmonary fibrosis is a serious respiratory disease, with limited therapeutic options. Since TGF-β is a critical factor in the fibrotic process, downregulation of this cytokine has been considered a potential approach for disease treatment. Herein, we designed a new lung-targeted delivery technology based on the complexation of polymeric antisense oligonucleotides (pASO) and dimeric human β-defensin 23 (DhBD23). Antisense oligonucleotides targeting TGF-β mRNA were polymerized by rolling circle amplification and complexed with DhBD23. After complexation with DhBD23, pASO showed improved serum stability and enhanced uptake by fibroblasts in vitro and lung-specific accumulation upon intravenous injection in vivo. The pASO/DhBD23 complex delivered into the lung downregulated target mRNA, and subsequently alleviated lung fibrosis in mice, as demonstrated by western blotting, quantitative reverse-transcriptase PCR (qRT-PCR), immunohistochemistry, and immunofluorescence imaging. Moreover, as the complex was prepared only with highly biocompatible materials such as DNA and human-derived peptides, no systemic toxicity was observed in major organs. Therefore, the pASO/DhBD23 complex is a promising gene therapy platform with lung-targeting ability to treat various pulmonary diseases, including pulmonary fibrosis, with low side effects.
URI
http://pubs.kist.re.kr/handle/201004/72160
ISSN
0168-3659
Appears in Collections:
KIST Publication > Article
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