Identifying potential biomarkers related to pre-term delivery by proteomic analysis of amniotic fluid

Title
Identifying potential biomarkers related to pre-term delivery by proteomic analysis of amniotic fluid
Authors
이지은홍수빈김유미박예현최지웅박교훈
Issue Date
2020-11
Publisher
Scientific Reports
Citation
VOL 10, NO 1-19648-10
Abstract
We sought to identify biomarkers in the amniotic fuid (AF) and specifc signaling pathways related to spontaneous preterm delivery (SPTD, < 34 weeks) in women with preterm labor (PTL) without intra-uterine infection/infammation (IUI). This was a retrospective cohort study of a total of 139 PTL women with singleton gestation (24 + 0 to 32 + 6 weeks) who underwent amniocentesis and who displayed no evidence of IUI. A nested case?control was conducted using pooled AF samples (n= 20) analyzed via label-free liquid chromatography-tandem mass spectrometry. In the total cohort, an ELISA validation study was performed for seven candidate proteins of interest. Proteomic analysis identifed 77 diferentially expressed proteins (DEPs, P< 0.05) in the AF from SPTD cases compared to term delivery controls. ELISA validation confrmed that women who had an SPTD before 34 weeks had signifcantly independently lower levels of VEGFR-1 and higher levels of lipocalin-2 and the Fc fragment of IgG binding protein in the AF. Five principle pathways associated with the 77 DEPs were identifed, including glycolysis, gluconeogenesis, and iron homeostasis. The proteomic analysis data of AFs from women with PTL identifed several novel biomarkers and specifc protein pathways related to SPTD in the absence of IUI.
URI
http://pubs.kist.re.kr/handle/201004/72246
ISSN
2045-2322
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KIST Publication > Article
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