Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin­derived peptide

Title
Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectin­derived peptide
Authors
박현미이경은박상은Gyu­Bum YeonHui­Gwan GooDong Sik SeoAhmed A. DayemSsang­Goo ChoDae­Sung Kim
Issue Date
2020-09
Publisher
Journal of cellular physiology
Citation
VOL 2020, NO 온라인게재-11
Abstract
Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, long­term cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectin­derived peptide­mussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNm­coated PVDF scaffold (VNm?PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm?PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiac­related genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm?PVDF scaffolds may be suitable for the long­term culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.
URI
http://pubs.kist.re.kr/handle/201004/72382
ISSN
0021-9541
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KIST Publication > Article
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