Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectinderived peptide
- Maintenance and differentiation of human ES cells on polyvinylidene fluoride scaffolds immobilized with a vitronectinderived peptide
- 박현미; 이경은; 박상은; GyuBum Yeon; HuiGwan Goo; Dong Sik Seo; Ahmed A. Dayem; SsangGoo Cho; DaeSung Kim
- Issue Date
- Journal of cellular physiology
- VOL 2020, NO 온라인게재-11
- Polyvinylidene fluoride (PVDF) is biocompatible, easy to fabricate, and has piezoelectric properties; it has been used for many biomedical applications including stem cell engineering. However, longterm cultivation of human embryonic stem cells (hESCs) and their differentiation toward cardiac lineages on PVDF have not been investigated. Herein, PVDF nanoscaled membrane scaffolds were fabricated by electrospinning; a vitronectinderived peptidemussel adhesive protein fusion (VNm) was immobilized on the scaffolds. hESCs cultured on the VNmcoated PVDF scaffold (VNm？PVDF scaffold) were stably expanded for more than 10 passages while maintaining the expression of pluripotency markers and genomic integrity. Under cardiac differentiation conditions, hESCs on the VNm？PVDF scaffold generated more spontaneously beating colonies and showed the upregulation of cardiacrelated genes, compared with those cultured on Matrigel and VNm alone. Thus, VNm？PVDF scaffolds may be suitable for the longterm culture of hESCs and their differentiation into cardiac cells, thus expanding their application in regenerative medicine.
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