Use of Elastic, Porous, and Ultrathin Co-Culture Membranes to Control the Endothelial Barrier Function via Cell Alignment
- Use of Elastic, Porous, and Ultrathin Co-Culture Membranes to Control the Endothelial Barrier Function via Cell Alignment
- 김수현; 정영미; 정지홍; 박상운; 김태희; 유진; 차국헌
- Issue Date
- Advanced functional materials
- VOL 31, NO 9, 2008172
- Porous membranes used in coculture enable the in vitro partitioning of cellular microenvironments, while still permitting physical and biochemical crosstalk between cells. Thus, features of the coculture membrane are crucial for recapitulating the physiological functions of cocultured cells. This study presents elastic, porous, and ultrathin membranes (EPUMs), which enhance cell？cell interactions and control cell alignment with surface topology created by stretching the membranes. The EPUM is fabricated using poly(lactidecocaprolactone) as the base material, and the porous feature is endowed by a vaporinduced phase separation process induced by the presence of hygroscopic salt. Owing to its elastic property, the membrane can be stretched, and the deformed porous structures on the membrane surfaces act as nanostructured topographical cues, resulting in cell alignment. By coculturing human mesenchymal stem cells (hMSCs) and human umbilical vein endothelial cells (HUVECs) on the opposite sides of the membrane, rapid endothelialization occurs through the membranes, as compared to the commercial membranes. Furthermore, the stretched membranes induce the alignment of hMSCs and HUVECs and ultimately exhibit enhanced endothelial barrier function. The coculture membrane developed in this study may provide an effective tool for recapitulating endothelial basement membranes with a controllable endothelial barrier function.
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