Fully integrated time-gated 3D fluorescence imager for deep neural imaging

Title
Fully integrated time-gated 3D fluorescence imager for deep neural imaging
Authors
이창혁최재빈Adriaan J. TaalWilliam L. MengEric H. PollmannJohn W. StantonSajjad MoazeniLaurent C. MoreauxMichael L. RoukesKenneth L. Shepard
Issue Date
2020-08
Publisher
IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
Citation
VOL 14, NO 4-645
Abstract
This paper presents a device for time-gated fluorescence imaging in the deep brain, consisting of two on-chip laser diodes and 512 single-photon avalanche diodes (SPADs). The edge-emitting laser diodes deliver fluorescence excitation above the SPAD array, parallel to the imager. In the time domain, laser diode illumination is pulsed and the SPAD is time-gated, allowing a fluorescence excitation rejection up to O.D. 3 at 1 ns of time-gate delay. Each SPAD pixel is masked with Talbot gratings to enable the mapping of 2D array photon counts into a 3D image. The 3D image achieves a resolution of 40, 35, and 73 μm in the x, y, and z directions, respectively, in a noiseless environment, with a maximum frame rate of 50 kilo-frames-per-second. We present measurement results of the spatial and temporal profiles of the dual-pulsed laser diode illumination and of the photon detection characteristics of the SPAD array. Finally, we show the imager's ability to resolve a glass micropipette filled with red fluorescent microspheres. The system's 420 μm-wide cross section allows it to be inserted at arbitrary depths of the brain while achieving a field of view four times larger than fiber endoscopes of equal diameter.
URI
http://pubs.kist.re.kr/handle/201004/72675
ISSN
1932-4545
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KIST Publication > Article
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