Fully integrated time-gated 3D fluorescence imager for deep neural imaging
- Fully integrated time-gated 3D fluorescence imager for deep neural imaging
- 이창혁; 최재빈; Adriaan J. Taal; William L. Meng; Eric H. Pollmann; John W. Stanton; Sajjad Moazeni; Laurent C. Moreaux; Michael L. Roukes; Kenneth L. Shepard
- Issue Date
- IEEE TRANSACTIONS ON BIOMEDICAL CIRCUITS AND SYSTEMS
- VOL 14, NO 4-645
- This paper presents a device for time-gated fluorescence imaging in the deep brain, consisting of two on-chip laser diodes and 512 single-photon avalanche diodes (SPADs). The edge-emitting laser diodes deliver fluorescence excitation above the SPAD array, parallel to the imager. In the time domain, laser diode illumination is pulsed and the SPAD is time-gated, allowing a fluorescence excitation rejection up to O.D. 3 at 1 ns of time-gate delay. Each SPAD pixel is masked with Talbot gratings to enable the mapping of 2D array photon counts into a 3D image. The 3D image achieves a resolution of 40, 35, and 73 μm in the x, y, and z directions, respectively, in a noiseless environment, with a maximum frame rate of 50 kilo-frames-per-second. We present measurement results of the spatial and temporal profiles of the dual-pulsed laser diode illumination and of the photon detection characteristics of the SPAD array. Finally, we show the imager's ability to resolve a glass micropipette filled with red fluorescent microspheres. The system's 420 μm-wide cross section allows it to be inserted at arbitrary depths of the brain while achieving a field of view four times larger than fiber endoscopes of equal diameter.
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