Assay Systems for Profiling Deubiquitinating Activity

Title
Assay Systems for Profiling Deubiquitinating Activity
Authors
김은경박진영조진홍송은주
Issue Date
2020-08
Publisher
International journal of molecular sciences
Citation
VOL 21, 5638
Abstract
Deubiquitinating enzymes regulate various cellular processes, particularly protein degradation, localization, and protein?protein interactions. The dysregulation of deubiquitinating enzyme (DUB) activity has been linked to several diseases; however, the function of many DUBs has not been identified. Therefore, the development of methods to assess DUB activity is important to identify novel DUBs, characterize DUB selectivity, and profile dynamic DUB substrates. Here, we review various methods of evaluating DUB activity using cell lysates or purified DUBs, as well as the types of probes used in these methods. In addition, we introduce some techniques that can deliver DUB probes into the cells and cell-permeable activity-based probes to directly visualize and quantify DUB activity in live cells. This review could contribute to the development of DUB inhibitors by providing important information on the characteristics and applications of various probes used to evaluate and detect DUB activity in vitro and in vivo.
URI
http://pubs.kist.re.kr/handle/201004/72677
ISSN
1661-6596
Appears in Collections:
KIST Publication > ETC
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