A Surface Tension Magnetophoretic Device for Rare Cell Isolation and Characterization

Title
A Surface Tension Magnetophoretic Device for Rare Cell Isolation and Characterization
Authors
임성환Emma E. van der ToomJames E. VerdoneChanghan JunDoru PetrisorJean J. M. C. H. de la RosetteTheo M. de ReijkeMichael A. GorinDan Stoianovici
Issue Date
2017-01
Publisher
MEDICAL ONCOLOGY
Citation
VOL 34, NO 2-819
Abstract
The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a 'real-time liquid biopsy'. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive 'lab on a chip' device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.
URI
http://pubs.kist.re.kr/handle/201004/72841
ISSN
1357-0560
Appears in Collections:
KIST Publication > Article
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