A 3D disease and regeneration model of peripheral nervous system?on?a?chip

Title
A 3D disease and regeneration model of peripheral nervous system?on?a?chip
Authors
김홍남Sujin HyungSeung-Ryeol LeeJiho KimYoungtaek KimSuryong KimNoo Li Jeon
Issue Date
2021-01
Publisher
Science Advances
Citation
VOL 7, NO 5, eabd9749
Abstract
Demyelinating diseases involve loss of myelin sheaths and eventually lead to neurological problems. Unfortunately, the precise mechanisms remain unknown, and there are no effective therapies. To overcome these limitations, a reliable and physiologically relevant in vitro model is required. Here, we present a three-dimensional peripheral nervous system (PNS) microfluidic platform that recapitulates the full spectrum of myelination, demyelination, and remyelination using primary Schwann cells (SCs) and motor neurons (MNs). The platform enables reproducible hydrogel patterning and long-term stable coculture of MNs and SCs over 40 days in vitro based on three distinct design factors. Furthermore, the on-demand detachable substrate allows in-depth biological analysis. We demonstrated the possibility of mimicking segmental demyelination by lysophosphatidylcholine, and recovery of myelin structure by application of two drugs: benzatropine or methylcobalamin. This 3D PNS disease?on?a?chip may serve as a potential platform for understanding the pathophysiology of demyelination and screening drugs for remyelination.
URI
http://pubs.kist.re.kr/handle/201004/72866
ISSN
2375-2548
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE