DNA methylome signatures as epigenetic biomarkers of hexanal associated with lung toxicity

Title
DNA methylome signatures as epigenetic biomarkers of hexanal associated with lung toxicity
Authors
류재천조윤송미경
Keywords
DNA methylome; Epigenetics; Hexanal; Aldehyde; Lung toxicity
Issue Date
2021-02
Publisher
PeerJ
Citation
VOL 9, e10779
Abstract
Background. Numerous studies have investigated the relationship of environmental exposure, epigenetic effects, and human diseases. These linkages may contribute to the potential toxicity mechanisms of environmental chemicals. Here, we investigated the epigenetic pulmonary response of hexanal, a major indoor irritant, following inhalation exposure in F-344 rats. Methods. Based on DNA methylation profiling in gene promoter regions, we identified hexanal-characterized methylated sites and target genes using an unpaired t-test with a fold-change cutoff of ≥ 3.0 and a p-value < 0.05. We also conducted an integrated analysis of DNA methylation and mRNA expression data to identify core anti-correlated target genes of hexanal exposure. To further investigate the potential key biological processes and pathways of core DNA methylated target genes, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed. Results. Thirty-six dose-dependent methylated genes and anti-correlated target genes of DNA methylation and mRNA in lung tissue of hexanal exposed F-344 rats were identified. These genes were involved in diverse biological processes such as neuroactive ligand-receptor interaction, protein kinase cascade, and intracellular signaling cascade associated with pulmonary toxicity. These results suggest that novel DNA methylationbased epigenetic biomarkers of exposure to hexanal and elucidate the potential pulmonary toxicological mechanisms of action of hexanal.
URI
http://pubs.kist.re.kr/handle/201004/72878
ISSN
2167-8359
Appears in Collections:
KIST Publication > Article
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