Physiological activity of E. coli engineered to produce butyric acid
- Physiological activity of E. coli engineered to produce butyric acid
- 최재영; 함정엽; 박영태; 김태정; 이회석; 연영주; 최수인; 김나영; 김연란; 석영재
- E. coli Nissle1917; butyric acid; butyryl-CoA dehydrogenase (BCD); glucose; DSS-colitis model; anti-inflammatory
- Issue Date
- Microbial biotechnology
- VOL 0, NO 0-12
- Faecalibacterium prausnitzii (F. prausnitzii) is one of the most abundant bacteria in the human intestine, with its antiinflammatory effects establishing it as a major effector in human intestinal health. However, its extreme sensitivity to oxygen makes its cultivation and physiological study difficult. F. prausnitzii produces butyric acid, which is beneficial to human gut health. Butyric acid is a shortchain fatty acid (SCFA) produced by the fermentation of carbohydrates, such as dietary fibre in the large bowel. The genes encoding butyrylCoA dehydrogenase (BCD) and butyrylCoA:acetate CoA transferase (BUT) in F. prausnitzii were cloned and expressed in E. coli to determine the effect of butyric acid production on intestinal health using DSSinduced colitis model mice. The results from the E. coli Nissle 1917 strain, expressing BCD, BUT, or both, showed that BCD was essential, while BUT was dispensable for producing butyric acid. The effects of different carbon sources, such as glucose, Nacetylglucosamine (NAG), Nacetylgalactosamine (NAGA), and inulin, were compared with results showing that the optimal carbon sources for butyric acid production were NAG, a major component of mucin in the human intestine, and glucose. Furthermore, the antiinflammatory effects of butyric acid production were tested by administering these strains to DSSinduced colitis model mice. The oral administration of the E. coli Nissle 1917 strain, carrying the expression vector for BCD and BUT (EcNBCDBUT), was found to prevent DSSinduced damage. Introduction of the BCD expression vector into E. coli Nissle 1917 led to increased butyric acid production, which improved the strain’s healthbeneficial effects.
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