Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR­Mediated Autophagy Pathway in Mouse Cortical Astrocytes

Title
Antioxidant Compound, Oxyresveratrol, Inhibits APP Production through the AMPK/ULK1/mTOR­Mediated Autophagy Pathway in Mouse Cortical Astrocytes
Authors
남길수임혜원조윤정Md. Ataur Rahman
Issue Date
2021-03
Publisher
Antioxidants
Citation
VOL 10, NO 3, 408
Abstract
Oxyresveratrol (OxyR), a well­known polyphenolic phytoalexin, possesses a wide range of pharmacological and biological properties, comprising antioxidant, anti­inflammatory, free radical scavenging, anti­cancer, and neuroprotective activities. Autophagy is a cellular self­degradation system that removes aggregated or misfolded intracellular components via the autophagosome­ly­sosomal pathway. Astrocyte accumulation is one of the earliest neuropathological changes in Alzheimer’s disease (AD), and amyloid precursor protein (APP) is the hallmark of AD. OxyR could affect APP modulation via the autophagy pathway. Here, we have reported that OxyR promotes autophagy signaling and attenuates APP production in primary cortical astrocytes based on immunofluorescence and immunoblotting assay results. Co­treatment with the late­stage autophagy inhibitor chloroquine (CQ) and OxyR caused significantly higher microtubule­associated protein light chain 3 (LC3)­II protein levels and LC3 puncta counts, demonstrating that OxyR stimulated autophagic flux. We also found that OxyR significantly reduced the levels of the autophagy substrate p62/SQSTM1, and p62 levels were significantly augmented by co­treatment with OxyR and CQ, because of the impaired deficiency of p62 in autolysosome. Likewise, pretreatment with the autoph agy inhibitor, 3­methyladenine (3­MA), resulted in significantly fewer OxyR­induced LC3 puncta and lower LC3­II expression, suggesting that OxyR­mediated autophagy was dependent on the class III PI3­kinase pathway. In contrast, OxyR caused significantly lower LC3­II protein expression when pretreated with compound C, an AMP­activated protein kinase (AMPK) inhibitor, indicating that AMPK signaling regulated the OxyR­induced autophagic pathway. Additionally, co­treatment with OxyR with rapamycin intended to inhibit the mammalian target of rapamycin (mTOR) caused significantly l
URI
http://pubs.kist.re.kr/handle/201004/73017
ISSN
2076-3921
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KIST Publication > Article
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