Microbial biosensor for Salmonella using anti-bacterial antibodies isolated from human serum

Title
Microbial biosensor for Salmonella using anti-bacterial antibodies isolated from human serum
Authors
강민정Jun-Hee ParkJi-Hong BongJaeyong JungJeong Soo SungGa-Yeon LeeJae-Chul Pyun
Keywords
Anti-Salmonella antibody; Bacterial detection; ClearCoil; Impedance spectrometry
Issue Date
2021-03
Publisher
Enzyme and microbial technology
Citation
VOL 144, 109721
Abstract
In this work, we present a novel microbial biosensor for Salmonella based on impedance spectrometry by using isolated antibodies against a specific bacterial strain from human serum. Anti-Salmonella (or BL21(DE3)) antibodies were isolated from human serum using S. enteritidis (or BL21(DE3)) and the mutant strain ClearColi. After the purification steps, the purification yield of the antibodies was calculated to be 0.2 %. From the FACS analysis, the isolated anti-Salmonella antibodies were estimated to have more than 6-fold higher binding affinity for S. enteritidis compared to antibodies against other kinds of Gram-negative bacterial strains, including HB101, ClearColi, JM110, DH5α, and BL21(DE3). Finally, the anti-Salmonella antibodies isolated herein were used for bacterial detection using electrochemical biosensors based on impedance spectrometry and the Rct value of the antibodies was estimated for S. enteritidis from the Nyquist plot. The limit of detection of the isolated anti-Salmonella antibodies was estimated to be 1.0×103 cells/mL for S. enteritidis and 1.0×106 cells/mL for BL21(DE3), respectively.
URI
http://pubs.kist.re.kr/handle/201004/73048
ISSN
0141-0229
Appears in Collections:
KIST Publication > Article
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE