Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry

Title
Diagnosis of severe sepsis using phospholipids enzymatic assay based on cyclic voltammetry
Authors
강민정Jun-Hee ParkJong-Min ParkGa-Yeon LeeZhiquan SongJeong Soo SungNam Su KuEun Hye LeeKwang Hwan ParkMoo Suk ParkSang-Guk LeeJae-Chul Pyun
Keywords
Sepsis; Diagnosis; Phospholipid; Enzyme assay; Cyclic voltammetry; Graphite
Issue Date
2021-03
Publisher
Enzyme and microbial technology
Citation
VOL 144, 109728
Abstract
In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n=16), patients with systemic inflammatory response syndrome (SIRS, n=16) and severe sepsis patients (n=24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.
URI
http://pubs.kist.re.kr/handle/201004/73049
ISSN
0141-0229
Appears in Collections:
KIST Publication > Article
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